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multiplex immunofluorescence staining lunaphore comet  (Lunaphore)

 
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    Lunaphore multiplex immunofluorescence staining lunaphore comet
    STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex <t>immunofluorescence</t> (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.
    Multiplex Immunofluorescence Staining Lunaphore Comet, supplied by Lunaphore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+immunofluorescence+staining+lunaphore+comet/pmc12066534-89-0-3?v=Lunaphore
    Average 90 stars, based on 1 article reviews
    multiplex immunofluorescence staining lunaphore comet - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Selective inhibition of canonical STAT3 signaling suppresses K-ras mutant lung tumorigenesis and reinvigorates anti-tumor immunity"

    Article Title: Selective inhibition of canonical STAT3 signaling suppresses K-ras mutant lung tumorigenesis and reinvigorates anti-tumor immunity

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1575181

    STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex immunofluorescence (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.
    Figure Legend Snippet: STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex immunofluorescence (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.

    Techniques Used: Inhibition, Flow Cytometry, Expressing, Ex Vivo, Multiplex Assay, Immunofluorescence



    Similar Products

    multiplex immunofluorescence staining lunaphore comet  (Lunaphore)
    90
    Lunaphore multiplex immunofluorescence staining lunaphore comet
    STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex <t>immunofluorescence</t> (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.
    Multiplex Immunofluorescence Staining Lunaphore Comet, supplied by Lunaphore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+immunofluorescence+staining+lunaphore+comet/pmc12066534-89-0-3?v=Lunaphore
    Average 90 stars, based on 1 article reviews
    multiplex immunofluorescence staining lunaphore comet - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex immunofluorescence (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.

    Journal: Frontiers in Immunology

    Article Title: Selective inhibition of canonical STAT3 signaling suppresses K-ras mutant lung tumorigenesis and reinvigorates anti-tumor immunity

    doi: 10.3389/fimmu.2025.1575181

    Figure Lengend Snippet: STAT3 inhibition increases DC and Th1 proportion within the TIME. (A) Representative dot plots of myeloid cell flow cytometry analysis pre-gated on CD11c + cells; expression of Ly6C and CD11b is used to delineate classical type 1 DCs (cDC1s)/alveolar macrophages (AMs), cDC2s, and monocytic DCs (Mo-DCs), and is quantified in (B) as a percentage of CD45 + cells (N = 3-5). (C) Representative dot plots of lymphoid cell flow cytometry analysis with ex vivo restimulation by PMA/Ionomycin, pre-gated on CD3 + cells; expression of CD4 and IFNγ is used to mark T helper 1 (Th1) cells and is quantified in (D) as a percentage of CD4 + cells (N = 3-5). (E) Representative multiplex immunofluorescence (COMET) images for B cells (B220 + ); original magnification, 20X; scale bar is 100 µm. Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test.

    Article Snippet: Multiplex immunofluorescence staining (Lunaphore COMET) was performed on FFPE-derived sections as described in Wang et al. ( ).

    Techniques: Inhibition, Flow Cytometry, Expressing, Ex Vivo, Multiplex Assay, Immunofluorescence